Chemical and biological characteristics of hydrolysate of crucian carp swim bladder: Focus on preventing ulcerative colitis

Journal of Functional Foods

Guo-Xiang Wang¹  Feng-Jie Yu²  Ya-Ping Lu²  Jun Chen³  Chen Dai³  Lin Dai³  Chong Wang³  Xi-Nuo Li⁴ 

[1] Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, PR China;College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China;College of Life Sciences, Experimental Teaching Center of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China;;Key Lab of Drug Metabolism &

关键词: Hydrolysate;    Inflammation;    Nano LC-LTQ-Orbitrap;    Oxidative stress;    Ulcerative colitis;   

DOI  :  

来源: DOAJ

【 摘 要 】

The incidence of ulcerative colitis (UC) is increasing worldwide. Currently, no curative treatment is available. Finding a food-based method for prevention of UC would be significant. In this study, the hydrolysate of crucian carp swim bladder (HCSB) was prepared using a simulated gastrointestinal digestion. 178 peptides in HCSB were identified by nano liquid chromatography coupled with hybrid linear ion trap Orbitrap mass spectrometry. In a model of hydrogen peroxide-induced oxidative stress in an intestinal epithelial cell line (IEC-6), HCSB protects IEC-6 cells by increasing antioxidant enzyme activity and reducing the intracellular ROS content. HCSB also represses lipopolysaccharide-induced inflammation of IEC-6 cells. In vivo studies showed that prophylactic administration of HCSB reduces the severity of dextran sodium sulfate-induced UC in mice by reducing myeloperoxidase activity, production of proinflammatory cytokines, and by inhibiting activation of the NF-κB pathway. These results indicate that HCSB has the potential to prevent ulcerative colitis.

【 授权许可】

Unknown