Quorum Sensing Regulates the Hydrolytic Enzyme Production and Community Composition of Heterotrophic Bacteria in Coastal Waters

Frontiers in Microbiology

Claire Labry¹  Daniel Delmas¹  Catherine Dreanno²  Raphaël Lami³  Marion Urvoy⁴  Stéphane L’Helguen⁴ 

[1] Ifremer, DYNECO, Plouzané, France;Ifremer, RDT, Plouzané, France;Sorbonne Université, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM, USR 3579), Observatoire Océanologique de Banyuls, Banyuls-sur-Mer, France;Université de Bretagne Occidentale, CNRS, IRD, Ifremer, UMR 6539, Laboratoire des Sciences de l’Environnement Marin (LEMAR), Plouzané, France;

关键词: hydrolytic enzymes;    bacterial community composition;    quorum sensing;    N-acylhomoserine lactone;    coastal waters;   

DOI  :  10.3389/fmicb.2021.780759

来源: DOAJ

【 摘 要 】

Heterotrophic microbial communities play a central role in biogeochemical cycles in the ocean by degrading organic matter through the synthesis of extracellular hydrolytic enzymes. Their hydrolysis rates result from the community’s genomic potential and the differential expression of this genomic potential. Cell-cell communication pathways such as quorum sensing (QS) could impact both aspects and, consequently, structure marine ecosystem functioning. However, the role of QS communications in complex natural assemblages remains largely unknown. In this study, we investigated whether N-acylhomoserine lactones (AHLs), a type of QS signal, could regulate both hydrolytic activities and the bacterial community composition (BCC) of marine planktonic assemblages. To this extent, we carried out two microcosm experiments, adding five different AHLs to bacterial communities sampled in coastal waters (during early and peak bloom) and monitoring their impact on enzymatic activities and diversity over 48 h. Several specific enzymatic activities were impacted during both experiments, as early as 6 h after the AHL amendments. The BCC was also significantly impacted by the treatments after 48 h, and correlated with the expression of the hydrolytic activities, suggesting that changes in hydrolytic intensities may drive changes in BCC. Overall, our results suggest that QS communication could participate in structuring both the function and diversity of marine bacterial communities.

【 授权许可】

Unknown