期刊论文详细信息
Sensors
Characterization of Quorum Sensing and Quorum Quenching Soil Bacteria Isolated from Malaysian Tropical Montane Forest
关键词: Arthrobacter;    Bacillus;    liquid chromatography mass spectrometry (LC-MS);    N-acylhomoserine lactone;    N-dodecanoyl-L-homoserine lactone;    p-coumaroylhomoserine lactone;    Pseudomonas frederiksbergensis;    quorum quenching;    quorum sensing;    rapid resolution liquid chromatography (RRLC);   
DOI  :  10.3390/s120404846
来源: DOAJ
【 摘 要 】

We report the production and degradation of quorum sensing N-acyl-homoserine lactones by bacteria isolated from Malaysian montane forest soil. Phylogenetic analysis indicated that these isolates clustered closely to the genera of Arthrobacter, Bacillus and Pseudomonas. Quorum quenching activity was detected in six isolates of these three genera by using a series of bioassays and rapid resolution liquid chromatography analysis. Biosensor screening and high resolution liquid chromatography-mass spectrometry analysis revealed the production of N-dodecanoyl-L-homoserine lactone (C12-HSL) by Pseudomonas frederiksbergensis (isolate BT9). In addition to degradation of a wide range of N-acyl-homoserine lactones, Arthrobacter and Pseudomonas spp. also degraded p-coumaroyl-homoserine lactone. To the best of our knowledge, this is the first documentation of Arthrobacter and Pseudomonas spp. capable of degrading p-coumaroyl-homoserine lactone and the production of C12-HSL by P. frederiksbergensis.

【 授权许可】

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