LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan

Molecules

Mohammed S. Al-Sheraideh¹  Nezar Al-Bataineh²  Yousef Al-Dalahmeh³  Jamil N. Lahham⁴  Mahmoud A. Al-Qudah⁵  Sara S. Al-Balawi⁵  Idrees F. Al-Momani⁵  Sultan T. Abu Orabi⁵  Abdulraouf S. Mayyas⁶ 

[1] Chemistry Department, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 383, Dammam 31113, Saudi Arabia;College of Pharmacy, Al Ain University of Science and Technology, Abu Dhabi P.O. Box 6414, United Arab Emirates;Department of Applied Pharmaceutical Sciences, Faculty of Pharmacy, Isra University, Amman 11622, Jordan;Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid 21163, Jordan;Department of Chemistry, Faculty of Science, Yarmouk University, P.O. Box 566, Irbid 21163, Jordan;Department of Conservation Science, Queen Rania Faculty of Tourism and Heritage, The Hashemite University, Zarqa 13133, Jordan;

关键词: total phenolic;    flavonoid;    antioxidant properties;    Calotropis procera;    Peruglaria tomentosa;    Pentatropis spiralis;   

DOI  :  10.3390/molecules27030859

来源: DOAJ

【 摘 要 】

This study aimed to evaluate the antioxidant activity and total phenolic content (TPC) and total flavonoid content (TFC) of crude extracts obtained from three Asclepiadaceae species, namely, Calotropis procera L., Peruglaria tomentosa L., and Pentatropis spiralis (Forsk.) Decne. Both butanol and aq. methanol extracts of the three species showed the highest amount of phenol and flavonoid contents, which exhibited the greatest antioxidant activity in the scavenging of 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS), ferrous chelating effect (FIC), and hydroxyl radical (HDR) assays. Phytochemical screening of the extracts revealed the presence of alkaloids, tannins, sponins, flavonoids, terpenoids, and glycosides. LC-MS analysis was carried out to identify the major compounds from each crude extract. A total of 12 phenolic compounds in the extracts of the 3 species were identified and quantified, including 9 flavonoids, 2 hydroxybenzoic acids, and 3 hydroxycinnamic acids. The current study also revealed a good correlation between total phenolic contents and the observed antioxidant activity of the crude extracts.

【 授权许可】

Unknown