Determination of total phenolic content and antioxidant activitity of methanol extract of Maranta arundinacea L fresh leaf and tuber

International Pharmacy Conference UAD 2017 on Product Authentication: Key Factor in Quality Control of Pharmaceutical Products

Kusbandari, A.^1 ; Susanti, H.^1

Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia^1

关键词: Anti-oxidant activities;    Free radical oxidation;    Herbaceous plants;    M. arundinacea L;    Phenolic compounds;    total phenolic;    Total phenolic content;    UV-Vis spectrophotometers;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/259/1/012010/pdf
DOI  :  10.1088/1757-899X/259/1/012010

来源: IOP

【 摘 要 】

Maranta arundinacea L is one of herbaceous plants in Indonesia which have flavonoid content. Flavonoids has antioxidants activity by inhibition of free radical oxidation reactions. The study aims were to determination total phenolic content and antioxidant activity of methanol extract of fresh leaf and tuber of M. arundinacea L by UV-Vis spectrophotometer. The methanol extracts were obtained with maceration and remaseration method of fresh leaves and tubers. The total phenolic content was assayed with visible spectrophotometric using Folin Ciocalteau reagent. The antioxidant activity was assayed with 1,1-diphenyl-2-picrilhidrazil (DPPH) compared to gallic acid. The results showed that methanol extract of tuber and fresh leaf of M. arundinacea L contained phenolic compound with total phenolic content (TPC) in fresh tuber of 3.881±0.064 (% GAE) and fresh leaf is 6.518±0.163 (% b/b GAE). IC50value from fresh tuber is 1.780±0.0005 μg/mL and IC50fresh leaf values of 0.274±0.0004 μg/mL while the standard gallic acid is IC50of 0.640±0.0002 μg/mL.