Aglycone sterics-selective enzymatic glycan remodeling

iScience

Xiaojian Wang¹  Lin Ding²  Huangxian Ju³  Haiqi Wang⁴  Guyu Wang⁴  Anwen Mao⁴  Tong Zhong⁴  Yan Zhang⁴  Ran Xie⁵  Xinyu Chen⁵ 

[1] Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China;Corresponding author;Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China;State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China;State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China;

关键词: Biological sciences;    Biochemistry;    Glycobiology;    Enzyme engineering;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Precision remodeling of glycans in their native environments is pivotal for understanding glycan-mediated biological events and has important biotechnological implications in fields of clinical diagnosis, glyco-immune checkpoint therapy, and so forth. However, the influence of aglycone-steric diversity on the selectivity of glycan remodeling has been largely overlooked, limiting the application in complex biological scenarios. Here, we report the achievement of aglycone sterics-selective enzymatic glycan remodeling by controlled grafting of functional polymers from glycoenzyme. Through tuning polymer length, a series of enzyme-polymer composites with varying substrate permeability are prepared, which afford an activity pattern-based differentiation strategy for aglycone sterics. This leads to the implementation of glycolipid’s partner screening, and aglycone sterics-selective glycan remodeling in a complex biological environment. We further orchestrate the polymer length adjustment with external cues to regulate aglycone-steric selectivity in a multi-faceted fashion, resulting in an unexpected enhancement of glycolipid remodeling, and temporal control of glycan remodeling on live cells.

【 授权许可】

Unknown