Coumarins from Seseli devenyense Simonk.: Isolation by Liquid–Liquid Chromatography and Potential Anxiolytic Activity Using an In Vivo Zebrafish Larvae Model

International Journal of Molecular Sciences

SimonVlad Luca¹  Jarosław Widelski²  Monika Maciąg³  Barbara Budzyńska³  Krystyna Skalicka-Woźniak⁴  Adrianna Skiba⁴  Laurence Marcourt⁵  Jean-Luc Wolfender⁵ 

[1] Biothermodynamics, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany;Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 20-093 Lublin, Poland;Independent Laboratory of Behavioral Studies, Medical University of Lublin, 20-093 Lublin, Poland;Independent Laboratory of Natural Products Chemistry, Medical University of Lublin, 20-093 Lublin, Poland;School of Pharmaceutical Sciences, Institute of Pharmaceutical Sciences of Western Switzerland, IPSWS, University of Geneva, CMU, 1211 Geneva, Switzerland;

关键词: Seseli devenyense;    Apiaceae;    countercurrent chromatography;    central nervous system;    anxiety;    Danio rerio;   

DOI  :  10.3390/ijms22041829

来源: DOAJ

【 摘 要 】

Different types of anxiety disorders have become the number one mental health issue in developed countries. The search for new, safer and effective drug-like molecules among naturally derived substances faces two difficulties: an efficient method of isolation compounds with a high-purity and high-throughput animal model for activity assay. Thus, the aim of the present study was to isolate by liquid–liquid chromatography high-purity rare coumarins from the fruits of Seseli devenyense Simonk. and evaluate their anxiolytic effect (defined as reversed thimotaxis) using a 5-days post-fertilization (dpf) Danio rerio larvae model. Liquid–liquid chromatography enabled the isolation of one simple hydroxycoumarin (devenyol) and four pyranocoumarins (cis-khellactone, d-laserpitin, isolaserpitin and octanoyllomatin). The anxiolytic effect was defined as a decrease in the time spent in the boundaries of the living space (also described as reversed thigmotaxis). Our results show that all isolated courmarins exerted a significant influence on the anxiety behavior (anxiolytic activity) in the zebrafish larvae model. According to our knowledge, this is the first report of anxiolytic activity of pyranocoumarins and devenyol.

【 授权许可】

Unknown