International Journal of Molecular Sciences | |
Coumarins from Seseli devenyense Simonk.: Isolation by Liquid–Liquid Chromatography and Potential Anxiolytic Activity Using an In Vivo Zebrafish Larvae Model | |
SimonVlad Luca1  Jarosław Widelski2  Monika Maciąg3  Barbara Budzyńska3  Krystyna Skalicka-Woźniak4  Adrianna Skiba4  Laurence Marcourt5  Jean-Luc Wolfender5  | |
[1] Biothermodynamics, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany;Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 20-093 Lublin, Poland;Independent Laboratory of Behavioral Studies, Medical University of Lublin, 20-093 Lublin, Poland;Independent Laboratory of Natural Products Chemistry, Medical University of Lublin, 20-093 Lublin, Poland;School of Pharmaceutical Sciences, Institute of Pharmaceutical Sciences of Western Switzerland, IPSWS, University of Geneva, CMU, 1211 Geneva, Switzerland; | |
关键词: Seseli devenyense; Apiaceae; countercurrent chromatography; central nervous system; anxiety; Danio rerio; | |
DOI : 10.3390/ijms22041829 | |
来源: DOAJ |
【 摘 要 】
Different types of anxiety disorders have become the number one mental health issue in developed countries. The search for new, safer and effective drug-like molecules among naturally derived substances faces two difficulties: an efficient method of isolation compounds with a high-purity and high-throughput animal model for activity assay. Thus, the aim of the present study was to isolate by liquid–liquid chromatography high-purity rare coumarins from the fruits of Seseli devenyense Simonk. and evaluate their anxiolytic effect (defined as reversed thimotaxis) using a 5-days post-fertilization (dpf) Danio rerio larvae model. Liquid–liquid chromatography enabled the isolation of one simple hydroxycoumarin (devenyol) and four pyranocoumarins (cis-khellactone, d-laserpitin, isolaserpitin and octanoyllomatin). The anxiolytic effect was defined as a decrease in the time spent in the boundaries of the living space (also described as reversed thigmotaxis). Our results show that all isolated courmarins exerted a significant influence on the anxiety behavior (anxiolytic activity) in the zebrafish larvae model. According to our knowledge, this is the first report of anxiolytic activity of pyranocoumarins and devenyol.
【 授权许可】
Unknown