Purification of a synthetic pterocarpanquinone by countercurrent chromatography

Journal of the Brazilian Chemical Society

Silva, Alcides José M. da¹  Costa, Paulo Roberto R.¹  Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil¹  Netto, Chaquip Daher¹  Leitão, Gilda G.¹  Costa, Fernanda das Neves¹  Domingos, Jorge L. de Oliveira¹  Universidade Federal do Rio de Janeiro, Macaé, Brazil¹ 

关键词: countercurrent chromatography;    pterocarpanquinone;    flash chromatography;    distribution coefficient (KD);   

DOI  :  10.1590/S0103-50532012000600016

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Countercurrent chromatography (CCC) was employed as a useful, fast and economic alternative to conventional chromatography techniques for the purification of a synthetic pterocarpanquinone, LQB-118. The separation was performed in a two-step CCC with the solvent system hexane-chloroform-methanol-water 2:1.5:5:2 in both steps. Traditional purification of these reaction products by silica gel column chromatography demanded a large amount of solvent and time, besides allowing the irreversible adsorption of the compound in the column. The use of 1H NMR for the calculation of KD of target compound is proposed as an alternative for HPLC measurements.

【 授权许可】

Unknown