Purification of a synthetic pterocarpanquinone by countercurrent chromatography

Journal of the Brazilian Chemical Society

Fernanda Das Neves Costa¹  Alcides José M. Da Silva¹  Chaquip Daher Netto²  Jorge L. De Oliveira Domingos¹  Paulo Roberto R. Costa¹  Gilda G. Leitão¹ 

[1] ,Universidade Federal do Rio de JaneiroRio de Janeiro RJ ,Brazil

关键词: countercurrent chromatography;    pterocarpanquinone;    flash chromatography;    distribution coefficient (K D);   

DOI  :  10.1590/S0103-50532012000600016

来源: SciELO

【 摘 要 】

Countercurrent chromatography (CCC) was employed as a useful, fast and economic alternative to conventional chromatography techniques for the purification of a synthetic pterocarpanquinone, LQB-118. The separation was performed in a two-step CCC with the solvent system hexane-chloroform-methanol-water 2:1.5:5:2 in both steps. Traditional purification of these reaction products by silica gel column chromatography demanded a large amount of solvent and time, besides allowing the irreversible adsorption of the compound in the column. The use of ¹H NMR for the calculation of K D of target compound is proposed as an alternative for HPLC measurements.

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