The hyperbaric oxygen preconditioning-induced brain protection is mediated by a reduction of early apoptosis after transient global cerebral ischemia

Neurobiology of Disease

Gerhart Graupner¹  Jeffrey Chiu²  Dalia Corleone²  Neal Dach²  Elena Titova²  Jiping Tang²  Jennifer Zhang²  John H. Zhang²  Robert P. Ostrowski² 

[1] Department of Pediatrics, Loma Linda University, USA;Department of Physiology and Pharmacology, Loma Linda University, USA;

关键词: Hyperbaric oxygen preconditioning;    Annexin V;    Apoptosis;    Global cerebral ischemia;    BDNF;    p38;   

DOI  :  

来源: DOAJ

【 摘 要 】

We hypothesized that the brain-protective effect of hyperbaric oxygen (HBO) preconditioning in a transient global cerebral ischemia rat model is mediated by the inhibition of early apoptosis.One hundred ten male Sprague–Dawley (SD) rats (300–350 g body weight) were allocated to the sham group and three other groups with 10 min of four-vessel occlusion, untreated or preconditioned with either 3 or 5 hyperbaric oxygenations. HBO preconditioning improved neurobehavioral scores and reduced mortality, decreased ischemic cell change, reduced the number of early apoptotic cells and hampered a conversion of early to late apoptotic alterations. HBO preconditioning reduced the immunoreactivity of phosphorylated p38 in vulnerable neurons and increased the expression of brain derived neurotrophic factor (BDNF) in early stage post-ischemia. However, preconditioning with 3 HBO treatments proved less beneficial than with 5 HBO treatments.We conclude that HBO preconditioning may be neuroprotective by reducing early apoptosis and inhibition of the conversion of early to late apoptosis, possibly through an increase in brain BDNF level and the suppression of p38 activation.

【 授权许可】

Unknown