IN VITRO PROPERTIES OF NEURAL CREST-DERIVED MULTIPOTENT STEM CELLS FROM A BULGE REGION OF WHISKER FOLLICLE

Biotechnologia Acta

S. N. Novikova¹  A. E. Rodnichenko¹  R. G. Vasyliev²  D.A. Zubov²  G. M. Butenko²  I. F. Labunets² 

[1] Biotechnology laboratory «Ilaya regeneration», Medical company ilaya, Kyiv, Ukraine;State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv;

关键词: neural crest;    multipotent stem cells;    hair follicle;    clonogenicity;    sphere-formation capacity.;   

DOI  :  10.15407/biotech7.04.071

来源: DOAJ

【 摘 要 】

A culture method for multipotent neural crest-derived stem cell isolated from the bulge region of the hair follicle of whisker pad of adult mice has been described and their biological properties have been studied. It was shown that the cells possess a fibroblast-like morphology, they are nestin-positive and cytokeratin-negative, and also express the following surface markers: CD44, CD73, CD90 and Sca-1. This cell type shows the functional properties of stem cells in culture: clonogenicity, self-renewal, sphere-forming capacity and the ability to the directed multilineage differentiation. Due to these properties, neural crest-derived multipotent stem cells are promising for application in the regenerative medicine

【 授权许可】

Unknown