International Journal of Molecular Sciences | |
Isolation of Multipotent Nestin-Expressing Stem Cells Derived from the Epidermis of Elderly Humans and TAT-VHL Peptide-Mediated Neuronal Differentiation of These Cells | |
Hiroshi Kanno2  Atsuhiko Kubo2  Tetsuya Yoshizumi2  Taro Mikami1  | |
[1] Department of Plastic Surgery, Yokohama City University, Yokohama 236-0004, Japan; E-Mails:;Department of Neurosurgery, Yokohama City University, Yokohama 236-0004, Japan; E-Mails: | |
关键词: hair follicle; multipotent stem cells; nestin; neuronal differentiation; | |
DOI : 10.3390/ijms14059604 | |
来源: mdpi | |
【 摘 要 】
A specialized population of cells residing in the hair follicle is quiescent but shows pluripotency for differentiating into epithelial-mesenchymal lineage cells. Therefore, such cells are hoped to be useful as implantable donor cells for regenerative therapy. Recently, it was reported that intracellular delivery of TAT-VHL peptide induces neuronal differentiation of skin-derived precursors. In the present study, we successfully isolated multipotent stem cells derived from the epidermis of elderly humans, characterized these cells as being capable of sphere formation and strong expression of nestin, fibronectin, and CD34 but not of keratin 15, and identified the niche of these cells as being the outer root sheath of the hair follicles. In addition, we showed that TAT-VHL peptide induced their neuronal differentiation
【 授权许可】
CC BY
© 2013 by the authors; licensee MDPI, Basel, Switzerland
【 预 览 】
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