LC–MS3 Strategy for Quantification of Carbamazepine in Human Plasma and Its Application in Therapeutic Drug Monitoring

Molecules

Jing Huang¹  Dongxiao Ma¹  Zhengchao Ji¹  Haiwei Cao¹  Lei Yin²  Lei Zeng³ 

[1] Department of Clinical Laboratory, The First Hospital of Jilin University, Jilin University, Changchun 130021, China;School of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin 124221, China;The Bethune Institute of Epigenetic Medicine, The First Hospital of Jilin University, Jilin University, Changchun 130021, China;

关键词: LC-MS³;    carbamazepine;    therapeutic drug monitoring;   

DOI  :  10.3390/molecules27041224

来源: DOAJ

【 摘 要 】

This study developed a detection method based on the strategy of HPLC/MS³ and verified its suitability by quantifying carbamazepine in human plasma. The high-performance liquid chromatography–tandem mass spectrometry (HPLC/MS³) system was performed using a Shimadzu UFLC XR liquid chromatography and a SCIEX QTRAP^® 5500 linear ion trap triple quadrupole mass spectrometer. The specific operation was as follows: the sample protein was firstly precipitated using methanol, then carbamazepine and carbamazepine-D₂N¹⁵ were separated on an ACQUITY UPLC HSS T3 column using the gradient elution with solvent A (0.1% formic acid) and solvent B (0.1% formic acid in acetonitrile) at a flow rate of 0.25 mL/min. Each sample was run for 7 min. This method was validated for various parameters including accuracy, precision, selectivity, linearity, LLOQ, etc. Only 5 μL of sample plasma could obtain the result of LLOD 0.5 µg/mL. The intra-day and inter-day precision was <8.23%, and accuracy was between −1.74% and 2.92%. This method was successfully used for monitoring the blood concentration of epilepsy patients after carbamazepine treatment.

【 授权许可】

Unknown