Simultaneous Determination of Phenobarbital, Phenytoin, Carbamazepine and Carbamazepine-10,11-epoxide in Plasma of Epileptic Patients

Pharmaceutical Sciences

Shadi Shiva¹  Mohammad Barzegar²  Azam Samadi³  Abolghasem Jouyban³  Shahram Sadeghvand⁴  Maryam Khoubnasabjafari⁵ 

[1] Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.;Pediatric Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.;Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.;Student Research Committee, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.;Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Science, Tabriz, Iran.;

关键词: phenobarbital;    carbamazepine;    carbamazepine metabolite;    phenytoin;    hplc analysis;    therapeutic drug monitoring;   

DOI  :  10.15171/PS.2019.43

来源: DOAJ

【 摘 要 】

Background: Quantitative analyses of antiepileptic drugs are required in clinic and to rational dosage adjustment, the clinician needs the blood levels of these drugs. A high-performance liquid chromatography with spectrophotometric detection has been developed and validated for simultaneous determination of some antiepileptic drugs in plasma of patients with epilepsy. Methods: A simple procedure based on deproteinization by acetonitrile was used for pre-treatment of plasma samples. Liquid chromatographic analysis was carried out on a Nova-Pak® C18 analytical column, using a ternary mixture of potassium dihydrogen phosphate buffer (pH 6.0)-acetonitrile-2-propanol (63:22:15, v/v/v) as the mobile phase, at a flow rate of 1.0 mL min-1. Results: Calibration curves were linear over a range of 1–40 µg mL-1 for phenobarbital, 1–30 µg mL-1 for phenytoin, 0.3–15 µg mL-1 for carbamazepine and 0.5–6 µg mL-1 for carbamazepine epoxide. Conclusion: The simple sample pre-treatment, combined with the fast chromatographic run was used for the determination of antiepileptic drugs for a large number of patient samples.

【 授权许可】

Unknown