Fibronectin fibers are highly tensed in healthy organs in contrast to tumors and virus-infected lymph nodes

Matrix Biology Plus

Martin Behe¹  Alessandra Moscaroli¹  Daniela Jaramillo²  Annette Oxenius²  Viola Vogel³  Simon Arnoldini³  Charlotte M. Fonta³ 

[1] Center for Radiopharmaceutical Sciences, Paul Scherrer Institute, CH-5232 Villigen, Switzerland;Institute of Microbiology, ETH Zürich, CH-8093 Zurich, Switzerland;Laboratory of Applied Mechanobiology, Institute of Translational Medicine, Department of Health Sciences and Technology, ETH Zurich, CH-8093 Zurich, Switzerland;

关键词: Extracellular matrix;    Fibronectin;    Mechanobiology;    Cancer;    Lymph node;    Virus infection;   

DOI  :  

来源: DOAJ

【 摘 要 】

The extracellular matrix (ECM) acts as reservoir for a plethora of growth factors and cytokines some of which are hypothesized to be regulated by ECM fiber tension. Yet, ECM fiber tension has never been mapped in healthy versus diseased organs. Using our recently developed tension nanoprobe derived from the bacterial adhesin FnBPA5, which preferentially binds to structurally relaxed fibronectin fibers, we discovered here that fibronectin fibers are kept under high tension in selected healthy mouse organs. In contrast, tumor tissues and virus-infected lymph nodes exhibited a significantly higher content of relaxed or proteolytically cleaved fibronectin fibers. This demonstrates for the first time that the tension of ECM fibers is significantly reduced upon pathological tissue transformations. This has wide implications, as the active stretching of fibronectin fibers adjusts critical cellular niche parameters and thereby tunes the reciprocal cell-ECM crosstalk. Mapping the tensional state of fibronectin fibers opens novel and unexpected diagnostic opportunities.

【 授权许可】

Unknown