Matrix assembly of recombinant fibronectin polypeptide consisting of amino‐terminal 70 kDa and carboxyl‐terminal 37 kDa regions

FEBS Letters

Maeda, Toshinaga¹  Sekiguchi, Kiyotoshi¹  Titani, Koiti¹  Ichihara-Tanaka, Keiko¹ 

[1] Institute for Comprehensive Medical Science, Fujita Health University School of Medicine, Toyoake, Aichi 470-11, Japan

关键词: Fibronectin;    Extracellular matrix;    Fibrillogenesis;   

DOI  :  10.1016/0014-5793(92)80236-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three different forms of recombinant human fibronectin polypeptides consisting of the amino-terminal 70 kDa region, the carboxyl-terminal 37 kDa region, or both, were expressed in mouse L cells. Although either the amino-terminal or the carboxyl-terminal region alone was only poorly incorporated into the extracellular matrix, the fused form of the polypeptide was highly capable of assembling into the matrix. These results indicate that matrix assembly of fibronectin requires both regions and can proceed in the absence of most of the type III repeats including the one containing the cell adhesive Arg-Gly-Asp sequence.

【 授权许可】

Unknown