Human amnion epithelial cells assemble tenascins and three fibronectin isoforms in the extracellular matrix

FEBS Letters

Zardi, Luciano¹  Linnala, Auli²  Balzac, Enrica¹  Virtanen, Ismo² 

[1] Laboratory of Cell Biology, Istituto Nazionale per la Ricerca sul Cancro, Viale Benedetto XV, 10, 16132 Genoa, Italy;Department of Anatomy, University of Helsinki, Siltavuorenpenger 20 A, 00170 Helsinki, Finland

关键词: Amnion epithelial cell;    Fibronectin;    Tenascin;    Extracellular matrix;    Secretion;   

DOI  :  10.1016/0014-5793(93)81494-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monoclonal antibodies (MAb) were used to show that cultured human amnion epithelial (HuA) cells produce tenascins (Tn) and isoforms of cellular fibronectin (cFn). Tn polypeptides of M_r 280,000 and 190,000, assembled into extracellular matrix (ECM) but not secreted into the culture medium by HuA cells, were electrophoretically similar to those produced by human fibroblasts as revealed with domain-specific MAbs. The results suggested that most Fn produced by HuA cells contained the extradomain (ED) A and an oncofetal domain but only a minor fraction EDB. In immunofluorescence Tn and Fn were seen in different cytoplasmie granules upon monensin-induced intracellular accumulation. Tn appeared to be deposited in the ECM in colocalization with Fn but distinctly slower. The present results show that cultured normal human epithelial cells synthesize Tn and three isoforms of cFn and secrete them by using different cytoplasmie pathways.

【 授权许可】

Unknown