Isoforms of cellular fibronectin and tenascin in amniotic fluid

FEBS Letters

von Koskull, Harriet²  Linnala, Auli¹  Virtanen, Ismo¹ 

[1] Department of Anatomy, University of Helsinki, PO Box 9, Siltavuorenpenger 20 A, FIN-00014 University of Helsinki, Helsinki, Finland;Departments I and II of Obstetrics and Gynecology, Helsinki University Central Hospital, 00290 Helsinki, Finland

关键词: Amniotic fluid;    Fibronectin;    Plasma;    Tenascin;    Isoform;    Extracellular matrix;   

DOI  :  10.1016/0014-5793(94)80266-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Amniotic fluid (AF) obtained from second trimester pregnancies presented extradomain (ED) A, B and an oncofetal (onc-f) domain containing isoforms of cellular fibronectin (cFn) in Western blotting of gelatin-bound polypeptides and directly of AF. Western blotting after sequential immunoprecipitation suggested at least three Fn molecules: one containing EDA and the onc-f domain and another minor component distinctly containing all the domains, and a third one only containing EDA. The immunoblotting results for EDA-cFn and onc-f-cFn were closely similar to that for total Fn, whereas in plasma samples of normal and pregnant women only traces of EDA-cFn and onc-f-cFn, but no EDB-cFn, were found. Western blotting of AF also indicated the presence of three isoforms of tenascin (Tn), M _r 190,000 and 280,000 polypeptides earlier found in many cells, and a M _r 200,000 polypeptide, novel for AF and not present in plasma. The results suggest a novel extracellular matrix polypeptide composition for AF.

【 授权许可】

Unknown