Simultaneous determination of related substances of oxytetracycline in pharmaceutical semi-solid form by HPLC method

Medicamentul Veterinar

关键词: simultaneous determination;    oxytetracycline;    4-epioxytetracycline;    alpha-apooxytetracycline;    tetracycline;    beta-apooxytetracycline;    HPLC method;   

DOI  :  

来源: DOAJ

【 摘 要 】

Simultaneous determination of oxytetracycline, 4-epioxytetracycline, alpha-apooxytetracycline, tetracycline, beta-apooxytetracycline on C18 columns has been accomplished using a high performance liquid chromatographic method with UV detection. Separation was achieved on a Hypersil BDS RP-C18 column with a mobile phase consisted of acetonitrile-methanol-80 mM dipotassium phosphate pH 7,5 at a flow rate of 0,7 mL min-1. Absorbance measurements were held at 254 nm.

【 授权许可】

Unknown