APPLICATION OF HPLC IN THE OXYTETRACYCLINE LATENCY PERIODESTABLISHMENT IN THE SHRIMP MUSCLE (Litopenaeus vannamei)IN A SEMI-INTESIVE PRODUCTION SYSTEM IN CUBA

Revista de Salud Animal

Escobar, A¹  Sosa, Dayana¹  Betancourt, A¹  Hernández, Damaiky²  Faure, R¹ 

[1] Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba;Grupo Empresarial para el Desarrollo del Cultivo de Camarón (GEDECAM)

关键词: oxytetracycline;    HPLC;    shrimp;    latency period  ;    INTRODUCCIÓ;    NLa acuacultura en Cuba constituye un regló;    n importante en los planes de incrementar la seguridad alimentaria y en la exportació;    n hacia diferentes mercados en Europa;   

DOI  :  

学科分类：兽医学

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

A methodology for determining of oxytetracycline in shrimp muscle by high performance liquid chromatography was developed using reverse phase column and UV detection at 340 nm. Extraction was done with a solution Mcllvaine-EDTA and the extracts purified using solid phase extraction columns (C18-6mL-500mg). The method showed a recovered between 78.2 ± 9.9% with a precision lesser than 10% in the concentrations of 200 and 400 ng/g, linearity showed a correlation coefficient higher than 0.99 and the detection limit was 105 ng/g. Oxytetracycline latency period in shrimp muscle (Litopenaeus vannamei) was set between 14-20 days when the medicated feed containing 4g/kg was supplied for 14 days under semi intensive production conditions. The analyses of residues in five shrimp farm at the harvest time showed oxytetracycline levels above 100 ng/g.

【 授权许可】

CC BY-NC