Revista de Salud Animal | |
APPLICATION OF HPLC IN THE OXYTETRACYCLINE LATENCY PERIODESTABLISHMENT IN THE SHRIMP MUSCLE (Litopenaeus vannamei)IN A SEMI-INTESIVE PRODUCTION SYSTEM IN CUBA | |
Escobar, A1  Sosa, Dayana1  Betancourt, A1  Hernández, Damaiky2  Faure, R1  | |
[1] Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba;Grupo Empresarial para el Desarrollo del Cultivo de Camarón (GEDECAM) | |
关键词: oxytetracycline; HPLC; shrimp; latency period INTRODUCCIÓ NLa acuacultura en Cuba constituye un regló n importante en los planes de incrementar la seguridad alimentaria y en la exportació n hacia diferentes mercados en Europa; | |
DOI : | |
学科分类:兽医学 | |
来源: Centro Nacional de Sanidad Agropecuaria | |
【 摘 要 】
A methodology for determining of oxytetracycline in shrimp muscle by high performance liquid chromatography was developed using reverse phase column and UV detection at 340 nm. Extraction was done with a solution Mcllvaine-EDTA and the extracts purified using solid phase extraction columns (C18-6mL-500mg). The method showed a recovered between 78.2 ± 9.9% with a precision lesser than 10% in the concentrations of 200 and 400 ng/g, linearity showed a correlation coefficient higher than 0.99 and the detection limit was 105 ng/g. Oxytetracycline latency period in shrimp muscle (Litopenaeus vannamei) was set between 14-20 days when the medicated feed containing 4g/kg was supplied for 14 days under semi intensive production conditions. The analyses of residues in five shrimp farm at the harvest time showed oxytetracycline levels above 100 ng/g.
【 授权许可】
CC BY-NC
【 预 览 】
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