Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood

Heliyon

Nguyen Doan Nguyen Phuong¹  Do Thi Phuong Linh²  Nguyen Bao Quoc³  Nguyen Ngoc Bao Chau³ 

[1] Corresponding author.;Faculty of Biotechnology, Ho Chi Minh City Open University, Vietnam;Research Institute for Biotechnology and Environment, Nong Lam University, Ho Chi Minh City, Vietnam;

关键词: Medicine;    Evidence-based medicine;    Infectious disease;    Pathology;    Biochemistry;    Bioengineering;   

DOI  :  

来源: DOAJ

【 摘 要 】

Recently, many studies have demonstrated the significant advantages of loop-mediated isothermal amplification (LAMP) based methods over serological tests and PCR for rapid detection of microbial pathogens. Here, a rapid LAMP assay was developed to detect the hepatitis B virus (HBV) from DNA, and particularly, blood samples from infected patients using a commercially available master mix and portable real-time fluorometer. The final optimized fluorescence-based LAMP assay provided significant amplification time of less than 15 minutes compared with over 1 hour for PCR and an opened tube LAMP system described previously. Results indicated that fluorescence-based LAMP assay was more sensitive than PCR as a rapid, sensitive, efficient, and highly reliable approach for rapid detection of HBV.

【 授权许可】

Unknown