Quantitative Detection of Plasmodium falciparum Using, LUNA-FL, A Fluorescent Cell Counter

Microorganisms

Toshihiro Mita¹  Kazumichi Yokota²  Masatoshi Kataoka²  Muneaki Hashimoto²  Kazuaki Kajimoto²  Noboru Minakawa³  Hiroaki Oka⁴  Atsuro Tatsumi⁴  Musashi Matsumoto⁴  Tomonori Hyodo⁵  Kiichiro Matsushita⁵  Kenichi Yamamoto⁵ 

[1] Department of Tropical Medicine and Parasitology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan;Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, Kagawa 761-0301, Japan;Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan;Konica Minolta, 1 Sakura-mashi, Hino, Tokyo 191-8511, Japan;Nitto Denko Corporation, 18, Hirayama, Nakahara-cho, Toyohashi, Aichi 441-3194, Japan;

关键词: malaria;    diagnosis;    fluorescent cell counter;    parasitemia;   

DOI  :  10.3390/microorganisms8091356

来源: DOAJ

【 摘 要 】

The microscopic examination of Giemsa-stained thin and/or thick blood films (Giemsa microscopy) is the standard method of malaria diagnosis. However, the results of the diagnosis significantly depend on the skills of clinical technicians. Furthermore, sample preparation and analysis are laborious and time-consuming. Therefore, in this study, we investigated if a commercially available fluorescent cell counter, LUNA-FL, was useful for the detection of Plasmodium parasite and the estimation of parasitemia. Whole blood samples from uninfected persons, spiked with P. falciparum-infected erythrocytes, were analysed. Most of the leucocytes and platelets were removed from whole blood samples with SiO₂-nanofiber filters set on spin columns. The filtered samples were stained with acridine orange, and automatic detection, as well as counting of erythrocytes and parasites, were performed using LUNA-FL. Whole blood, with various levels of parasites, was analysed by Giemsa microscopy or with LUNA-FL to estimate parasitemia, and a comparative analysis was performed. The coefficient determination value of the regression line was high (R² = 0.98), indicating that accurate quantitative parasite detection could be performed using LUNA-FL. LUNA-FL has a low running cost; it is compact, fast, and easy to operate, and may therefore be useful for point-of-care testing in the endemic areas.

【 授权许可】

Unknown