Highly Sensitive and Rapid Quantitative Detection of Plasmodium falciparum Using an Image Cytometer

Microorganisms

Toshihiro Mita¹  Yoshihiro Nakajima²  Kazumichi Yokota²  Masatoshi Kataoka²  Muneaki Hashimoto²  Kazuaki Kajimoto²  Noboru Minakawa³  Hiroaki Oka⁴  Atsuro Tatsumi⁴  Musashi Matsumoto⁴ 

[1] Department of Tropical Medicine and Parasitology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan;Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, Kagawa 761-0301, Japan;Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan;Konica Minolta, 1 Sakura-mashi, Hino, Tokyo 191-8511, Japan;

关键词: malaria;    diagnosis;    image cytometer;    parasitemia;   

DOI  :  10.3390/microorganisms8111769

来源: DOAJ

【 摘 要 】

The gold standard for malaria diagnosis is microscopic examination of blood films by expert microscopists. It is important to detect submicroscopic and asymptomatic Plasmodium infections in people, therefore the development of highly sensitive devices for diagnosing malaria is required. In the present study, we investigated whether an imaging cytometer was useful for the highly sensitive quantitative detection of parasites. Whole blood samples were prepared from uninfected individuals spiked with Plasmodium falciparum-infected erythrocytes. Thereafter, erythrocytes were purified using a push column comprising of a syringe filter unit with SiO₂-nanofiber filters. After adding the erythrocytes, stained with nuclear stain, to a six-well plate, quantitative detection of the parasites was performed using an image cytometer, CQ1. Imaging of 2.6 × 10⁶ erythrocytes was completed in 3 min, and the limit of detection indicated parasitemia of 0.00010% (≈5 parasites/μL of blood). In addition to rapid, highly sensitive, and quantitative detection, the ease of application and economic costs, image cytometry could be efficiently applied to diagnose submicroscopic parasites in infected people from endemic countries.

【 授权许可】

Unknown