Use of nested PCR for the detection of trichomonads in bronchoalveolar lavage fluid

BMC Infectious Diseases

Furong Ying¹  Xiaolong Li¹  Dongwei Hu¹  Tieli Zhou¹  Chao Lin¹  Yanan Lai¹  Xiangyang Xue² 

[1] The First Affiliated Hospital of Wenzhou Medical University;Wenzhou Medical University;

关键词: Bronchoalveolar lavage fluid;    Nested PCR;    Trichomonad;    Lung;   

DOI  :  10.1186/s12879-019-4118-9

来源: DOAJ

【 摘 要 】

Abstract Background The methods routinely used to detect trichomonads in the lungs are not sensitive enough, and an effective method is urgently needed. Method Primers were first designed to match the conserved area of the 18S rRNA gene of trichomonads. Then, nested PCR was carried out to detect trichomonads in bronchoalveolar lavage fluid (BALF). Finally, all positive specimens were subjected to DNA sequencing and phylogenetic analysis. Results Among 115 bronchoalveolar lavage fluid samples, ten samples tested positive in nested PCR (10/115), while no samples were positive in wet mount microscopy (0/115) (P < 0.01). Among the ten positive specimens, two were identified as Tetratrichomonas spp. and the other eight as Trichomonas tenax in phylogenetic analysis. Conclusions Nested PCR is an effective way to detect trichomonads in bronchoalveolar lavage fluid.

【 授权许可】

Unknown