An easy and robust method for the isolation of high quality RNA from coconut tissues

Electronic Journal of Biotechnology

Jing Li¹  Anwar Hussain¹  Yaodong Yang²  Yi Wu³  Amjad Iqbal⁴  Muhammad Hamayun⁴  Farooq Shah⁴ 

[1] Corresponding authors.;;Department of Food Science &Technology, Abdul Wali Khan University Mardan, 23200, Pakistan;Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Science, Wenchang, Hainan 571339, China;

关键词: cDNA library;    Coconut tissues;    Complex matrices;    High quality RNA;    RNA extraction;    RT-PCR;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: Coconut tissues consist of a complex network of polysaccharides, proteins, polyphenols, and lipids that can bind to nucleic acids and pose difficulty in isolation. Certainly, a vigorous method is required to isolate high quality and quantity of RNA from such tissues for the purpose of downstream experiments. In this paper, we discuss a newly developed method for the Isolation of RNA from Complex Matrices (IRCM) method from coconut tissues. Results: The method is robust, cheap, and efficient for the extraction of quality RNA in high quantities from the solid endosperm of stored and fresh coconut (150 μg/g FW with A260/280 = 1.89 and 247.5 μg/g FW with A260/280 = 1.91), coconut apple (263.8 μg/g FW with A260/280 = 1.97), and coconut bud (1052.5 μg/g FW with A260/280 = 2.00). The other well established methods, such as Method of RNA Isolation from Palm (MRIP), Cetyl Trimethyl Ammonium Bromide (CTAB), TRIZOL, and RNA plant kit failed to isolate quality RNA in appreciable quantities from the coconut tissues. Furthermore, the resultant RNA performed well in the downstream experiment, that is, RT-PCR for the production and amplification of cDNA. Conclusions: From the study, we concluded that the present method will play a vital role in the extraction of high quality RNA from complex matrices in a short time.How to cite: Iqbal A, Yang Y, Wu Y, et al. An easy and robust method for the isolation of high quality RNA from coconut tissues. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.09.008

【 授权许可】

Unknown