Generation and Analysis of Expressed Sequence Tags (ESTs) from Halophyte Atriplex canescens to Explore Salt-Responsive Related Genes

International Journal of Molecular Sciences

Jingtao Li¹  Xinhua Sun¹  Gang Yu¹  Chengguo Jia¹  Jinliang Liu¹ 

[1] College of Plant Science, Jilin University, Changchun 130062, China;

关键词: Atriplex canescens;    cDNA library;    expressed sequence tags;    salt stress tolerance;    SSRs;   

DOI  :  10.3390/ijms150611172

来源: mdpi

【 摘 要 】

Little information is available on gene expression profiling of halophyte A. canescens. To elucidate the molecular mechanism for stress tolerance in A. canescens, a full-length complementary DNA library was generated from A. canescens exposed to 400 mM NaCl, and provided 343 high-quality ESTs. In an evaluation of 343 valid EST sequences in the cDNA library, 197 unigenes were assembled, among which 190 unigenes (83.1% ESTs) were identified according to their significant similarities with proteins of known functions. All the 343 EST sequences have been deposited in the dbEST GenBank under accession numbers JZ535802 to JZ536144. According to Arabidopsis MIPS functional category and GO classifications, we identified 193 unigenes of the 311 annotations EST, representing 72 non-redundant unigenes sharing similarities with genes related to the defense response. The sets of ESTs obtained provide a rich genetic resource and 17 up-regulated genes related to salt stress resistance were identified by qRT-PCR. Six of these genes may contribute crucially to earlier and later stage salt stress resistance. Additionally, among the 343 unigenes sequences, 22 simple sequence repeats (SSRs) were also identified contributing to the study of A. canescens resources.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.