Molecular cloning of a full‐length cDNA encoding the hemagglutinin‐neuraminidase glycoprotein of Sendai virus

FEBS Letters

Miura, Naoyuki²  Uchida, Tsuyoshi²  Okada, Yoshio²  Nakatani, Yoshihiro²  Ishiura, Masahiro¹ 

[1]National Institute for Basic Biology, Okazaki, Aichi 444, Japan

[2]Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamada-Oka, Suita, Osaka 565 Japan

关键词: Recombinant DNA;    cDNA library;    Oligodeoxynucleotide;    Sendai virus;    Hemagglutinin-neuraminidase protein;    Nucleotide sequence;   

DOI  :  10.1016/0014-5793(85)80885-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We cloned a full-length complementary DNA for the hemagglutinin-neuraminidase (HN) mRNA of Sendai virus (HVJ) using a synthetic 27-mer as a probe. Nucleotide sequence analysis showed that there is a long open reading frame on the mRNA that encodes a protein of 575 amino acids. The deduced amino acid sequence indicated that only one hydrophobic region sufficiently long to anchor the protein in the membrane and located near the N-terminus (amino acids 35–60). It is suggested that HN protein is oriented with its N-terminus inside the membrane.

【 授权许可】

Unknown