Purification of Recombinant Galectins Expressed in Bacteria

STAR Protocols

Julieta Carabelli¹  María Virginia Tribulatti²  Valentina Cattaneo²  Oscar Campetella²  Cecilia Arahí Prato² 

[1] Corresponding author;Laboratorio de Inmunología Molecular, Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Martín, Buenos Aires B1650HMP, Argentina;

关键词: Cell Biology;    Molecular Biology;    Protein Biochemistry;    Protein expression and purification;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Galectins are soluble lectins that participate in many physiological and pathological functions. Since they can act extracellularly, the use of the recombinant protein is a recurrent strategy for studying their biological functions. Here, we provide a general protocol for the production of Galectins and their isolated or chimeric domains. We take advantage of their lectin activity and the 6xHis-tag addition for purification, thus obtaining a highly pure and active Galectin to use in both in vitro and in vivo assays.For complete details on the use and execution of this protocol, please refer to Cattaneo et al. (2011), Tribulatti et al. (2012), and Prato et al. (2020).

【 授权许可】

Unknown