Generation of recombinant vaccinia virus and analysis of virus-induced cell death

STAR Protocols

Kidong Kang¹  Zhijun Liu²  Francis Ka-Ming Chan² 

[1] Corresponding author;Department of Immunology, Duke University School of Medicine, DUMC 3010, Durham, NC 27710, USA;

关键词: Cell Biology;    Immunology;    Microbiology;    Molecular Biology;    Protein expression and purification;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Vaccinia virus is a large double-stranded DNA virus that is widely used to express foreign genes from different origins. We generated recombinant vaccinia virus that expresses a viral inhibitor to examine its effect on virus-induced necroptosis. We provide a detailed protocol to describe the generation of recombinant vaccinia virus, validation of protein expression, and determination of necroptosis using live cell imaging. This approach can be adapted to examine the effect of other cell death regulators on virus-induced cell death.For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).

【 授权许可】

Unknown