Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes

South African Journal of Chemical Engineering

Sinouvassane Djearamane¹  Sharolynne Xiao Tong Liang²  Poh Foong Lee³  Yang Mooi Lim⁴  Ling Shing Wong⁵ 

[1] Biomedical Engineering, Lee Kong Chian Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, Bandar Sungai Long 43000, Malaysia;Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar 31900, Malaysia;;Department of Mechatronics &Department of Pre-Clinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Bandar Sungai Long 43000, Malaysia;Life Science Division, Faculty of Health and Life Sciences, INTI International University, Nilai 71800, Malaysia;

关键词: Antibacterial effects;    Bacterial growth inhibition;    Oxidative stress;    Streptococcus pyogenes;    Zinc oxide nanoparticles;   

DOI  :  

来源: DOAJ

【 摘 要 】

Zinc oxide nanoparticles (ZnO NPs) have been used in the treatment of bacterial infections due to their antibacterial properties. The present study was aimed to explore the antibacterial effects of ZnO NPs on Streptococcus pyogenes. The antibacterial properties were evaluated by investigating the growth inhibition and oxidative stress effects of ZnO NPs on S. pyogenes. The bacterial growth inhibition was determined through the percentage of reduction in the turbidity of bacterial suspension and bacterial colony upon treating with ZnO NPs, while the oxidative stress of ZnO NPs on bacterial cells were assessed by measuring the levels of reactive oxygen species (ROS) and lipid peroxidation. The results showed the dose dependent and significant (p < 0.05) growth inhibition and oxidative stress effects on S. pyogenes for 10 to 100 μg/mL ZnO NPs at 24 h. Fourier-transform infrared spectrometry (FTIR) analysis confirmed the binding of ZnO NPs to polypeptides and glycogen from the bacterial cell wall. Further, the scanning electron microscopy (SEM) results showed the attachment of ZnO NPs to bacterial cell wall and the resultant cell wall disruption and cell damage. The study results confirmed the antimicrobial effects of ZnO NPs on S. pyogenes.

【 授权许可】

Unknown