Three-Dimensional Culture Systems for Dissecting Notch Signalling in Health and Disease

International Journal of Molecular Sciences

Oriana Trubiani¹  Cristina Porcheri²  Guya Diletta Marconi²  Thimios A. Mitsiadis² 

[1] Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, Italy;Orofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, 8032 Zurich, Switzerland;

关键词: spheroids;    organoids;    organ-on-a-chip;    microfluidics;    3D culture systems;    Notch signalling;   

DOI  :  10.3390/ijms222212473

来源: DOAJ

【 摘 要 】

Three-dimensional (3D) culture systems opened up new horizons in studying the biology of tissues and organs, modelling various diseases, and screening drugs. Producing accurate in vitro models increases the possibilities for studying molecular control of cell–cell and cell–microenvironment interactions in detail. The Notch signalling is linked to cell fate determination, tissue definition, and maintenance in both physiological and pathological conditions. Hence, 3D cultures provide new accessible platforms for studying activation and modulation of the Notch pathway. In this review, we provide an overview of the recent advances in different 3D culture systems, including spheroids, organoids, and “organ-on-a-chip” models, and their use in analysing the crucial role of Notch signalling in the maintenance of tissue homeostasis, pathology, and regeneration.

【 授权许可】

Unknown