Polydatin Protects Bovine Mammary Epithelial Cells Against Zearalenone-Induced Apoptosis By Inhibiting Oxidative Responses and Endoplasmic Reticulum Stress

Toxins

AnShan Shan¹  JingLin Shen²  Hao Yu²  HengTong Fang²  YuRong Fu²  YongCheng Jin²  ChangHai Zhou²  JunXiong Wang²  Jing Zhang²  Yun Zhao²  Yue Tian²  HongYu Tang² 

[1] Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China;Key Laboratory of Zoonosis Research, Department of Animal Science, College of Animal Sciences, Jilin University, Ministry of Education, Changchun 130062, China;

关键词: Apoptosis;    bovine mammary epithelial cells;    endoplasmic reticulum stress;    polydatin;    zearalenone;   

DOI  :  10.3390/toxins13020121

来源: DOAJ

【 摘 要 】

Zearalenone (ZEA) is a mycotoxin of the Fusarium genus that can cause endoplasmic reticulum (ER) stress and Apoptosis in bovine mammary epithelial cells (MAC-T). Polydatin (PD), a glycoside purified from Polygonum cuspidatum, has antioxidant properties. This study aimed to explore whether PD can alleviate ZEA-induced damage on bovine mammary epithelial cells (MAC-T). We found that incasing the concentration of ZEA (0, 7.5, 15, 30, 60, 90, 120, and 240 μM) gradually decreased the cell viability. PD treatment alone at 5, 10, and 20 μM did not affect cell viability. Follow-up studies then applied 30 μM of ZEA and 5 μM of PD to treat cells; the results showed that the ZEA + PD treatment group effectively reduced cell oxidative damage compared with the ZEA treatment group. The qPCR analysis showed that ZEA treatment significantly up-regulated the expression of ER stress-related genes, relative to the control. However, adding PD significantly down-regulated the expression of ER stress-related genes. The cell apoptosis detection results showed that, compared with the ZEA treatment group, the ZEA + PD treatment group down-regulated the Bax gene and up-regulated the Bcl-2 gene expressions, which reduced the cell apoptosis rate and Caspase-3 activity. Taken together, these results indicate that PD reduces ZEA-induced apoptosis by inhibiting oxidative damage and ER stress.

【 授权许可】

Unknown