Enrichment of A Rare Subpopulation of miR-302-Expressing Glioma Cells by Serum Deprivation

Cell Journal

Sara Rohban¹  Seyed Javad Mowla¹  Afsaneh Malekzadeh Shafaroudi²  Mahmoud-Reza Rafiee³  Hamid Khayatzadeh³  Hamid Reza Kalhor⁴ 

[1] Department of Molecular Genetics, Faculty of Biological Sciences, T arbiat Modares University, Tehran, Iran;Nanomedicine and Tissue Engineering Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran,Department of Non-coding RNA Research, Pars Genome Company, Tehran, Iran;Nanomedicine and Tissue Engineering Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Department of Chemistry, Sharif University, Tehran, Iran;

关键词: Gene Expression;    microRNA;    miR-302;    Glioma;    Cancer Stem Cell;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objective: MiR-302-367 is a cluster of polycistronic microRNAs that are exclusively expressed in embryonic stem (ES) cells. The miR-302-367 promoter is functional during embryonic development but is turned off in later stages. Motivated by the cancer stem cell hypothesis, we explored the potential expression of miR-302 in brain tumor cell lines. Materials and Methods: In the present experimental study, we have tried to expand our knowledge on the expression pattern and functionality of miR302 cluster by quantifying its expression in a series of glioma (A-172, 1321N1, U87MG) and medulloblastoma (DAOY) cell lines. To further assess the functionality of miR-302 in these cell lines, we cloned its promoter core region upstream of the enhanced green fluorescent protein (EGFP) or luciferase encoding genes. Results: Our data demonstrated a very low expression of miR-302 in glioma cell lines, compared with that of embryonal carcinoma cell line NT2 being used as a positive control. The expression of miR-302 promoter-EGFP construct in the aforementioned cell lines demonstrated GFP expression in a rare subpopulation of the cells. Serum deprivation led to the generation of tumorospheres, enrichment of miR-302 positive cells and upregulation of a number of pluripotency genes. Conclusion: Taken together, our data suggest that miR-302 could potentially be used as a novel putative cancer stem cell marker to identify and target cancer stem cells within tumor tissues.

【 授权许可】

Unknown