Microfluidics for single-cell lineage tracking over time to characterize transmission of phenotypes in Saccharomyces cerevisiae

STAR Protocols

Diana Aguilar-Gómez¹  Igor Kukhtevich²  Robert Schneider³  Antonis Kirmizis⁴  Gilles Charvin⁵  Poonam Bheda⁶  Johannes Becker⁶ 

[1] Corresponding author;Center for Computational Biology, UC Berkeley, Berkeley, CA 94720, USA;Department of Biological Sciences, University of Cyprus, 2109 Nicosia, Cyprus;Development and Stem Cells, IGBMC, 67400 Illkirch, France;Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland;Institute of Functional Epigenetics, Helmholtz Zentrum München, 85764 Neuherberg, Germany;

关键词: Cell biology;    Single cell;    Microscopy;    Model organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The budding yeast Saccharomyces cerevisiae is an excellent model organism to dissect the maintenance and inheritance of phenotypes due to its asymmetric division. This requires following individual cells over time as they go through divisions to define pedigrees. Here, we provide a detailed protocol for collecting and analyzing time-lapse imaging data of yeast cells. The microfluidics protocol can achieve improved time resolution for single-cell tracking to enable characterization of maintenance and inheritance of phenotypes.For complete details on the use and execution of this protocol, please refer to Bheda et al. (2020a).

【 授权许可】

Unknown