Modifications to the classical rat aortic ring model to allow vascular degeneration studies

STAR Protocols

Thérèse-Marie Sartoris¹  Florian Sennlaub²  Guillaume Blot²  Xavier Guillonneau² 

[1] Corresponding author;Institut de la Vision, Sorbonne Université, INSERM, CNRS, Paris, France;

关键词: Cell biology;    Cell culture;    Model organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The classical aortic ring model is well suited for deciphering pro-angiogenic processes. Here, we propose simple modifications of the standard protocol to study various anti-angiogenic processes from growth arrest to capillary degeneration. Aortic rings are cultured under basal conditions for 6 days to allow physiological vessel sprouting and then split into treatment groups to follow capillary growth or degeneration for an additional 2 days.

【 授权许可】

Unknown