期刊论文详细信息
STAR Protocols | |
Modifications to the classical rat aortic ring model to allow vascular degeneration studies | |
Thérèse-Marie Sartoris1  Florian Sennlaub2  Guillaume Blot2  Xavier Guillonneau2  | |
[1] Corresponding author;Institut de la Vision, Sorbonne Université, INSERM, CNRS, Paris, France; | |
关键词: Cell biology; Cell culture; Model organisms; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Summary: The classical aortic ring model is well suited for deciphering pro-angiogenic processes. Here, we propose simple modifications of the standard protocol to study various anti-angiogenic processes from growth arrest to capillary degeneration. Aortic rings are cultured under basal conditions for 6 days to allow physiological vessel sprouting and then split into treatment groups to follow capillary growth or degeneration for an additional 2 days.
【 授权许可】
Unknown