C. elegans survival assays to discern global and transcription-coupled nucleotide excision repair

STAR Protocols

Melanie van der Woude¹  Hannes Lans¹ 

[1] Department of Molecular Genetics, Erasmus MC Cancer Institute, Erasmus University Medical Center, 3000 CA Rotterdam, the Netherlands;

关键词: Cell biology;    Genetics;    Model organisms;    Molecular biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Global genome nucleotide excision repair (GG-NER) and transcription-coupled nucleotide excision repair (TC-NER) protect cells against a variety of helix-distorting DNA lesions. In C. elegans, GG-NER primarily acts in proliferative germ cells and embryos, while TC-NER acts in post-mitotic somatic cells to maintain transcription. We leverage this difference to distinguish whether proteins function in GG-NER and/or TC-NER by straightforward UV survival assays. Here, we detail a protocol for these assays, using GG-NER factor xpc-1 and TC-NER factor csb-1 as examples.For complete details on the use and execution of this protocol, please refer to Sabatella et al. (2021).

【 授权许可】

Unknown