Visualization of cortical microtubule networks in plant cells by live imaging and immunostaining

STAR Protocols

Yuling Jiao¹  Feng Zhao²  Jan Traas²  Fei Du³ 

[1] Corresponding author;Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Inria, 69342 Lyon, France;State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China;

关键词: Cell biology;    Microscopy;    Model organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Cortical microtubules (CMTs) play pivotal roles during plant cell growth and division. The organization of CMTs undergoes important changes during different cellular and developmental processes. Here, we describe two methods for the visualization of CMT organization in plant cells using confocal laser scanning microscopy. CMT networks in the outer tissue layers can be directly visualized by live imaging of a fluorescent reporter line, and a protocol combining sectioning and immunostaining is applied for visualization of CMTs throughout tissues.For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).

【 授权许可】

Unknown