Identification of a Major QTL and Validation of Related Genes for Tiller Angle in Rice Based on QTL Analysis

International Journal of Molecular Sciences

Jae-Ryoung Park¹  Kyung-Hwan Kim²  Soo In Lee²  Gang-Seob Lee²  Xiao-Xuan Du²  Eun-Gyeong Kim³  Yoon-Hee Jang³  Muhammad Farooq³  Kyung-Min Kim³  Dan-Dan Zhao³  Rahmatullah Jan³ 

[1] Crop Breeding Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea;Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea;Department of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, Korea;

关键词: plant architecture;    rice;    tiller angle;    quantitative trait loci;    auxin;    breeding;   

DOI  :  10.3390/ijms23095192

来源: DOAJ

【 摘 要 】

An ideal plant architecture is an important condition to achieve high crop yields. The tiller angle is an important and complex polygenic trait of rice (Oryza sativa L.) plant architecture. Therefore, the discovery and identification of tiller angle-related genes can aid in the improvement of crop architecture and yield. In the present study, 222 SSR markers were used to establish a high-density genetic map of rice doubled haploid population, and a total of 8 quantitative trait loci (QTLs) were detected based on the phenotypic data of the tiller angle and tiller crown width over 2 years. Among them, four QTLs (qTA9, qCW9, qTA9-1, and qCW9-1) were overlapped at marker interval RM6235–RM24288 on chromosome 9 with a large effect value regarded as a stable major QTL. The selected promising related genes were further identified by relative gene expression analysis, which gives us a basis for the future cloning of these genes. Finally, OsSAURq9, which belongs to the SMALL AUXIN UP RNA (SAUR), an auxin-responsive protein family, was selected as a target gene. Overall, this work will help broaden our knowledge of the genetic control of tiller angle and tiller crown width, and this study provides both a good theoretical basis and a new genetic resource for the breeding of ideal-type rice.

【 授权许可】

Unknown