Overexpression of OsDof12 affects plant architecture in rice (Oryza sativa L.)

Frontiers in Plant Science

Xue eLiu¹  Xiaobing eLi²  Qi eWu²  Lihuang eZhu²  Xianfeng eZhao²  Dayong eLi²  Dejun eLi³  Shigui eLi⁴ 

[1] Beijing Institute of Genomics, Chinese Academy of Sciences;Institute of Genetics and Developmental Biology, Chinese Academy of Sciences;Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences;Rice Research Institute, Sichuan Agricultural University;

关键词: rice;    plant architecture;    Oryza sativa L.;    DOF transcription factor;    OsDof12;   

DOI  :  10.3389/fpls.2015.00833

来源: DOAJ

【 摘 要 】

Dof (DNA binding with one finger) proteins, a class of plant-specific transcription factors, are involved in plant growth and developmental processes and stress responses. However, their biological functions remain to be elucidated, especially in rice (Oryza sativa L.). Previously, we have reported that OsDof12 can promote rice flowering under long-day conditions. Here, we further investigated the other important agronomical traits of the transgenic plants overexpressing OsDof12 and found that overexpressing OsDof12 could lead to reduced plant height, erected leaf, shortened leaf blade, and smaller panicle resulted from decreased primary and secondary branches number. These results implied that OsDof12 is involved in rice plant architecture formation. Furthermore, we performed a series of Brassinosteroid (BR)-responsive tests and found that overexpression of OsDof12 could also result in BR hyposensitivity. Of note, in WT plants the expression of OsDof12 was found upregulated by BR treatment while in OsDof12 overexpression plants two positive BR signaling regulators, OsBRI1 and OsBZR1, were significantly down-regulated, indicating that OsDof12 may act as a negative BR regulator in rice. Taken together, our results suggested that overexpression of OsDof12 could lead to altered plant architecture by suppressing BR signaling. Thus, OsDof12 might be used as a new potential genetic regulator for future rice molecular breeding.

【 授权许可】

Unknown