Mapping quantitative trait loci associated with yield and yield components under reproductive stage salinity stress in rice (Oryza sativa L.)

Journal of genetics

Genaleen Q. Diaz¹  Merlyn S. Mendioro¹  Glenn B. Gregorio²  Reza Mohammadi²  Rakesh K. Singh1² 

[1] Genetics and Molecular Biology Division, Institute of Biological Sciences, University of the Philippines, Los Baños, Philippines$$;Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, DAPO, Box 7777, Metro Manila, Philippines$$

关键词: rice;    salinity tolerance;    reproductive stage;    quantitative trait loci;    grain yield.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Salinity tolerance in rice is critical at reproductive stage because it ultimately determines grain yield. An F2 mapping population derived from a Sadri/FL478 cross was exposed to saline field conditions (6–8 dS m-1) after the active tillering stage to identify reproductive stage specific QTLs for salinity tolerance. Genetic linkage map was constructed using 123 microsatellite markers on 232 F2 progenies. Totally 35 QTLs for 11 traits under salinity stress were detected with LOD >3, out of which 28 QTLs that explained from 5.9 to 30.0% phenotypic variation were found to be significant based on permutation test. Three major QTL clusters were found on chromosomes 2 (RM423–RM174), 4 (RM551–RM518) and 6 (RM20224–RM528) for multiple traits under salinity stress. Both parental lines contributed additively for QTLs identified for the yield components. A majority of the QTLs detected in our study are reported for the first time for reproductive stage salinity stress. Fine-mapping of selected putative QTLs will be the next step to facilitate marker-assisted backcrossing and to detect useful genes for salinity tolerance at the reproductive stage in rice.

【 授权许可】

Unknown