Gene Mapping of Attributed Traits to Salinity Tolerance at Seedling and Reproductive Stages in Rice

مجله بیوتکنولوژی کشاورزی

GHasem Mohammadinejad¹  Marjan GHasemkhani² 

[1] استادیار بخش زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان;دانشجوی کارشناسی ارشد بخش بیوتکنولوژی ، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان;

关键词: qtl mapping;    recombination inbred lines (rils);    rice;    salinity tolerance;   

DOI  :  10.22103/jab.2012.473

来源: DOAJ

【 摘 要 】

In order to identify the responsible QTLs attributed to salinity tolerance at seedling and reproductively stages in rice and detection of the ratio of each QTL in phenotypic variation, eighty RILs derived from IR29/Pokkali cross were used. The Sodium (Na) and Potassium (K) concentration, Na/K ratio at seedling stage and height, number and grain weight, number of panicle, number of tillers, fertility and biomass of rice in salinity stress at reproductive stage were assessed as phenotypic traits. Using 81 polymorphic SSR markers, a major QTL related to seedling stage tolerance was detected on the opposite arm of Saltol QTL in chromosome 1. Moreover, a major effected QTL for Na, K and their ratio was found on chromosomes 1 and 10 which totally controlled the tolerance at seedling stage. For tolerance to salinity at reproductive stage; major QTLs detected on chromosome 7, 9 and 10 for Reduction of Filled Grain Weight and Reduction of Biomass Weight . Multi trait QTL which were located on chromosome 10 had the same location with the QTL mapped for seedling stage tolerance, so it can be a good candidate for marker assisted breeding. It can be expected to achieve to the high tolerant genotypes through QTL pyramiding of saltol and chromosomal region of 10 together.

【 授权许可】

Unknown