Distinct gene expression signatures induced by viral transactivators of different HTLV-1 subgroups that confer a different risk of HAM/TSP

Retrovirology

Kazumasa Shirai¹  Kousuke Hanada¹  Yuetsu Tanaka²  Hiroshi Ushirogawa³  Tadasuke Naito³  Mineki Saito³  Hiroe Sejima³  Tatsufumi Nakamura⁴  Masahiro Fujii⁵  Jun-ichirou Yasunaga⁶  Yuichi Mitobe⁶  Masao Matsuoka⁶ 

[1] Department of Bioscience and Bioinformatics, Kyushu Institute of Technology;Department of Immunology, Graduate School of Medicine, University of the Ryukyus;Department of Microbiology, Kawasaki Medical School;Department of Social Work, Faculty of Human and Social Studies, Nagasaki International University;Division of Virology, Niigata University Graduate School of Medical and Dental Sciences;Laboratory of Virus Control, Institute for Frontier Life and Medical Sciences, Kyoto University;

关键词: HTLV-1;    Virus subgroup;    HAM/TSP;    Tax;    HBZ;    Microarray;   

DOI  :  10.1186/s12977-018-0454-x

来源: DOAJ

【 摘 要 】

Abstract Background Among human T cell leukemia virus type 1 (HTLV-1)-infected individuals, there is an association between HTLV-1 tax subgroups (subgroup-A or subgroup-B) and the risk of HAM/TSP in the Japanese population. To investigate the role of HTLV-1 subgroups in viral pathogenesis, we studied the functional difference in the subgroup-specific viral transcriptional regulators Tax and HBZ using microarray analysis, reporter gene assays, and evaluation of viral-host protein–protein interaction. Results (1) Transcriptional changes in Jurkat Tet-On human T-cells that express each subgroup of Tax or HBZ protein under the control of an inducible promoter revealed different target gene profiles; (2) the number of differentially regulated genes induced by HBZ was 2–3 times higher than that induced by Tax; (3) Tax and HBZ induced the expression of different classes of non-coding RNAs (ncRNAs); (4) the chemokine CXCL10, which has been proposed as a prognostic biomarker for HAM/TSP, was more efficiently induced by subgroup-A Tax (Tax-A) than subgroup-B Tax (Tax-B), in vitro as well as in unmanipulated (ex vivo) PBMCs obtained from HAM/TSP patients; (5) reporter gene assays indicated that although transient Tax expression in an HTLV-1-negative human T-cell line activated the CXCL10 gene promoter through the NF-κB pathway, there was no difference in the ability of each subgroup of Tax to activate the CXCL10 promoter; however, (6) chromatin immunoprecipitation assays showed that the ternary complex containing Tax-A is more efficiently recruited onto the promoter region of CXCL10, which contains two NF-κB binding sites, than that containing Tax-B. Conclusions Our results indicate that different HTLV-1 subgroups are characterized by different patterns of host gene expression. Differential expression of pathogenesis-related genes by subgroup-specific Tax or HBZ may be associated with the onset of HAM/TSP.

【 授权许可】

Unknown