Elevated cerebrospinal fluid and serum adenosine deaminase activities in experimental Listeria monocytogenes meningoencephalitis

Acta Veterinaria

关键词: adenosine deaminase;    cerebrospinal fluid;    Listeria monocytogenes;    rabbit;    serum;   

DOI  :  10.2298/AVB1206521Y

来源: DOAJ

【 摘 要 】

Listeriosis is a severe infectious disease occurring both in humans and inmost other animal species caused by Listeria monocytogenes (L. monocytogenes)that can lead to severe meningoencephalitis. Elevated ADA activities havebeen demonstrated in the cerebrospinal fluid (CSF) in meningitis withdifferent etiologies. However, data on ADA activities in CSF and serumassociated with listerial meningoencephalitis is limited. Thus, the presentstudy was designed to investigate the changes in the CSF and serum ADAactivities and whether there is a correlation between CSF and serum ADA inthe experimental model of listeriosis. Twenty adult white New Zealand rabbitsof both sexes were used in this study. Fourteen rabbits were infected byintracerebral inoculation of L. monocytogenes 1/2a and 6 healthy rabbits wereincluded in the study as the control group. CSF samples were obtained bypuncture of the cisterna magna. Blood was sampled from the auricular vein ofrabbits. ADA activity was determined using the colorimetric method. Mean±SDADA activities in the CSF and serum were 14.98±3.26 U/L and 17.71±3.41 U/L ininfected rabbits, respectively, while 4.72±1.86 U/L and 6.89±3.00 U/L inhealthy rabbits. CSF and serum ADA activities showed significant alterationin rabbits with experimental listeriosis compared to control rabbits(p<0.001). A positive correlation was found between CSF and serum ADAactivities in infected rabbits (r=0.65, p<0.05), but not in healthy rabbits.These findings imply that ADA plays a role in this disease process. Inconclusion, CSF and serum ADA measurements may be used as a supplementarybiochemical test in combination with clinical and laboratory findings oflisterial meningoencephalitis.

【 授权许可】

Unknown