Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens

Memórias do Instituto Oswaldo Cruz

Rafaela Moledo De Vasconcelos¹  Antonio Eugenio Castro Cardoso De Almeida¹  Ernesto Hofer²  Nicéa Magaly Matias Da Silva¹  Victor Augustus Marin¹ 

[1] ,FIOCRUZ Instituto Nacional de Controle e Qualidade em Saúde Laboratório de Zoonoses BacterianasRio de Janeiro RJ ,Brasil

关键词: Listeria monocytogenes;    listeriosis;    Multiplex-PCR;    cerebrospinal fluid;   

DOI  :  10.1590/S0074-02762008000800016

来源: SciELO

【 摘 要 】

The genus Listeria is composed of six species of which Listeria monocytogenes is considered the single pathogenic species that causes listeriosis in humans. Of the 13 serovars of L. monocytogenes, 1/2a, 1/2b and 4b are responsible for the majority of clinical cases. The aim of this work was to detect L. monocytogenes in the cerebrospinal fluid sample of premature newborns and to characterize this sample using biotyping, serotyping and molecular typing. The results indicated the presence of L. monocytogenesin the clinical sample studied. Moreover, the isolate was identified as the 4b serovar that was characterized by the presence of a unique 691 bp band after analysis using the Multiplex-PCR technique. The results of repeated Multiplex-PCR and sequencing have indicated that the L. monocytogenes isolate was an atypical 4b serovar, which is the first time this finding has been reported.

【 授权许可】

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