Inhibitory activity of bacteriocin produced from Lactobacillus SCG 1223 toward L. monocytogenes, S. thypimurium and E. coli

International Symposium on Food and Agro-biodiversity 2017

农业科学;生物科学

Marwati, T.^1 ; Cahyaningrum, N.^1 ; Widodo, S.^1 ; Januarsyah, T.^2 ; Purwoko^2

Assessment Institute for Agricultural Technology Yogyakarta, Indonesia^1

Faculty of Agricultural Technology, Bogor Agricultural University, Indonesia^2

关键词: Bactericidal ability;    Bacteriocins;    Completely randomized designs;    Incubation temperatures;    Inhibitory activity;    Lactobacillus SCG1223;    Listeria monocytogenes;    Pathogenic bacterium;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/102/1/012091/pdf
DOI  :  10.1088/1755-1315/102/1/012091

来源: IOP

【 摘 要 】

Bacteriocin is a protein compound which has bactericidal ability against pathogen bacteria. This research aims to study the inhibitory activity of bacteriocin produced from Lactobacillus SCG 1223 against Listeria monocytogenes, Salmonella thypimuruim and Escherchia coli. The bacteriocin produce from Lactobacillus SCG 1223 in the MRS broth media The experimental design used was Completely Randomized Design. The variations used in this design were percentage of inoculum (5%, 10%), medium pH (4, 6), incubation temperature (27°C, 40°C), and incubation time (4, 10, 14 hours). Result showed that bacteriocin from Lactobacillus SCG 1223 had wide spectrum toward L. monocytogenes, S. thypimuruim and E. coli. The highest bacteriocin activity toward L. monocytogenes produced by Lactobacillus SCG 1223 with 10% inoculum in media with initial pH 6, incubation temperature 27°C for 14 hour, toward S. thypimurium produced by Lactobacillus SCG 1223 with in media with initial pH 6, incubation temperature 40°C for 14 hour, and toward E. coli was 1085.81 AU/ml, produced by Lactobacillus SCG 1223 in MRS broth with initial pH 4, incubation temperature 40°C for 14 hour. This study is expected to find a new food preservative that can inhibit the growth of pathogenic bacteria and extend the shelf life of food. From the economic prospective of view, bacteriocin is very promising natural alternative biopreservatives.