Effects of Cultivating Conditions on the Water Soluble Polysaccharides Content of Ganoderma lucidum Mycelium in Submerged Flask Culture

International Conference on Natural Products and Bioresource Science 2017

生物科学;化学

Rosyida, V.T.^1 ; Hayati, S.N.^1 ; Apriyana, W.^1 ; Darsih, C.^1 ; Hernawan^1 ; Poeloengasih, C.D.^1

Research Unit for Natural Product Technology, Indonesian Institute of Sciences, (BPTBA-LIPI), Jl. Jogja-Wonosari Km 31.5, Gading, Playen, Gunung Kidul, D.I Yogyakarta

55861, Indonesia^1

关键词: Erlenmeyer flasks;    Fruiting bodies;    Ganoderma Lucidum;    Incubation temperatures;    Metabolite production;    Optimum temperature;    Potato dextrose agars;    Water-soluble polysaccharides;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/101/1/012008/pdf
DOI  :  10.1088/1755-1315/101/1/012008

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

The carcinostatic substance in Ganoderma lucidum (Fr.) Karst (Polyporaceae) is a water soluble polysaccharides (WSP) which might be useful in immunotherapy. Attempt to produce effective substances from cultured mycelia is important to carry out since solid cultivation is a time consuming and quality fluctuating. The effects of cultivating conditions on the water soluble polysaccharides content of G. Lucidum mycelium were investigated in submerged flask cultures. Culture from fruiting bodies was maintained on potato dextrose-agar slope. Slopes were inoculated and incubated at 30°C for 7 days, and stored at 4°C. The flask experiments were performed in 100 ml erlenmeyer flasks containing 20 ml of the sterilized media. Actively growing mycelia (1 piece, 5 mm X 5 mm) from a newly prepared slant culture (about 7 days incubation at 30°C) were inoculated into the flask. The pH was measured and adjusted to the desired value by addition of either 4 M HCl or 2.5 M NaOH. Incubation temperature were 20, 25, and 30°C. At the end of inoculation period (14 days) mycelium consisting of individual pellets was harvested and wash for the analysis. WSP content was analysed using phenol-sulfuric acid method. The optimal initial pH for metabolite production would depend on the culture medium. Generally, high values of pH, such as 9, negatively affect both cell growth and WSP production. The optimum temperature range for the high G. lucidum mycelium and WSP production were found to be 25 - 30 °C at pH values 5 - 7 in both of media.