Intestinal Microbiota and Serum Metabolic Profile Responded to Two Nutritional Different Diets in Mice

Frontiers in Nutrition

Miaomiao Dong¹  Shiyu Tao¹  Hong Wei¹  Hang Zhang¹  Zhifeng Wu¹  Xiang Tan¹  Yuqing Wang¹  Huicong Zou¹  Yapeng Yang¹  Wei Cheng¹  Shuaifei Feng¹  Yingping Xiao²  Zhenyu Wang³ 

[1] College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China;

关键词: germ-free diet;    nutrient;    microbiota;    metabolism;    gut development;   

DOI  :  10.3389/fnut.2021.813757

来源: DOAJ

【 摘 要 】

There is an interaction and bidirectional selection between dietary intake and gut microbiota due to the different efficiency of nutrients in the gut. The nutritional composition of germ-free (GF) diets differs significantly from specific pathogen-free (SPF) diets. There is, however, no data revealing how SPF animals from the same microbial background respond to them and if they affect the host. We examined the growth of SPF mice on the GF diet and found that it reduced body weight, intestinal length and intestinal morphology. Interestingly, the GF diet increased the level of pro-inflammatory bacteria in the gut of SPF mice, including Proteobacteria, Burkholderiaceae, Alloprevotella and Parasutterella. Furthermore, GF diets caused significant increases in malondialdehyde (MDA), IL-1β, IL-6, and D-lactate levels in the serum of SPF mice and significantly altered their serum metabolic profile, especially amino acid metabolism. In conclusion, GF diets are not suitable for the growth and development of SPF mice. These findings, based on the role of gut microbiota in diet selection, provide new insights into the scientific and rational use of experimental animal diets.

【 授权许可】

Unknown