Química Nova | |
Determination of fipronil in bovine plasma by solid-phase extraction and liquid chromatography with ultraviolet detection | |
Yara P. Cid1  Thais P. Ferreira1  Nathália C. C. Silva1  Viviane S. Magalhães1  Fábio B. Scott1  Deborah M. V. C. Medeiros1  Rodrigo M. Oliveira1  | |
[1] Universidade Federal Rural do Rio de Janeiro; | |
关键词: fipronil; bovine plasma; LC-UV; | |
DOI : 10.1590/S0100-40422012001000029 | |
来源: DOAJ |
【 摘 要 】
A fast and efficient method has been developed and validated for the determination of fipronil in bovine plasma. Samples were subjected to solid-phase extraction (SPE) followed by reversed phase liquid chromatography (LC) separation, using acetonitrile/water (60:40 v/v) as the mobile phase with a flow rate of 1.0 mL/min and ultraviolet (UV) detection at 210 nm. Ethiprole was used as the internal standard (IS). The method was found to be linear over the range 5-500 ng/mL (r = 0.999). The limit of quantitation (LOQ) was validated at 5 ng/mL. The method was successfully applied to monitor plasma concentrations following subcutaneous administration of fipronil in cattle.
【 授权许可】
Unknown