Determination of fipronil in bovine plasma by solid-phase extraction and liquid chromatography with ultraviolet detection

Química Nova

Yara P. Cid²  Thais P. Ferreira²  Deborah M. V. C. Medeiros²  Rodrigo M. Oliveira²  Nathália C. C. Silva²  Viviane S. Magalhães¹  Fábio B. Scott² 

[1] ,Universidade Federal Rural do Rio de Janeiro Instituto de Ciências Exatas Departamento de QuímicaSeropédica RJ ,Brasil

关键词: fipronil;    bovine plasma;    LC-UV;   

DOI  :  10.1590/S0100-40422012001000029

来源: SciELO

【 摘 要 】

A fast and efficient method has been developed and validated for the determination of fipronil in bovine plasma. Samples were subjected to solid-phase extraction (SPE) followed by reversed phase liquid chromatography (LC) separation, using acetonitrile/water (60:40 v/v) as the mobile phase with a flow rate of 1.0 mL/min and ultraviolet (UV) detection at 210 nm. Ethiprole was used as the internal standard (IS). The method was found to be linear over the range 5-500 ng/mL (r = 0.999). The limit of quantitation (LOQ) was validated at 5 ng/mL. The method was successfully applied to monitor plasma concentrations following subcutaneous administration of fipronil in cattle.

【 授权许可】

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