Determination of fipronil in bovine plasma by solid-phase extraction and liquid chromatography with ultraviolet detection

Química Nova

Ferreira, Thais P.¹  Magalhães, Viviane S.¹  Scott, Fábio B.¹  Universidade Federal Rural do Rio de Janeiro, Seropédica, Brasil¹  Silva, Nathália C. C.¹  Medeiros, Deborah M. V. C.¹  Cid, Yara P.¹  Oliveira, Rodrigo M.¹ 

关键词: fipronil;    bovine plasma;    LC-UV.;   

DOI  :  10.1590/S0100-40422012001000029

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A fast and efficient method has been developed and validated for the determination of fipronil in bovine plasma. Samples were subjected to solid-phase extraction (SPE) followed by reversed phase liquid chromatography (LC) separation, using acetonitrile/water (60:40 v/v) as the mobile phase with a flow rate of 1.0 mL/min and ultraviolet (UV) detection at 210 nm. Ethiprole was used as the internal standard (IS). The method was found to be linear over the range 5-500 ng/mL (r = 0.999). The limit of quantitation (LOQ) was validated at 5 ng/mL. The method was successfully applied to monitor plasma concentrations following subcutaneous administration of fipronil in cattle.

【 授权许可】

Unknown